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. 2015 Feb 11;10(2):e0118190. doi: 10.1371/journal.pone.0118190

Fig 4. ATG5 mediates sertraline-induced autophagy in htRPE cells.

Fig 4

(A and B) htRPE/GFP-LC3 cells were transfected with scrambled siRNA (Sc) or a specific siRNA to ATG5. After 3 days, the cells were further treated with sertraline (10 μM) for 24 h. Cells with autophagy were determined by counting the punctate GFP-LC3 dots under a florescence microscope (A). The protein expression levels of LC3 and ATG5 were examined by Western blot analysis (B). (C) The m5-7 MEF cells were treated with Sertraline (10 μM) in the presence or absence of doxycycline (Dox, 10 ng/mL) for 24 h. Then, expression of ATG5 and LC3 was analyzed by Western blotting. (D and E) htRPE/GFP-LC3 cells pre-treated with 3MA (5 mM) for 12 h were treated with sertraline (10 μM) for 24 hr. And cells with autophagy and LC3 protein were examined. Data represent ± standard error of the mean (S.E.M.) from more than three independent experiments (n > 3, * p < 0.05).