Table 4. FCS Autocorrelation analysis of ECFP-tubulin in PTK2 cells treated with microtubule depolymerizing agents.
No treatment | Nocodazole | S-methyl DM1 | Demecolcine | Vinblastine | |
---|---|---|---|---|---|
Molecular number | 8 ± 1 | 17 ± 3 | 18 ± 3 | 11 ± 3 | 6 ± 1 |
Dwell time, ms | 3.0 ± 0.2 | 3.6 ± 0.5 | 3.2 ± 0.2 | 3.0 ± 0.3 | 5.8 ± 0.4 |
Number of cells | 29 | 12 | 9 | 9 | 11 |
Each compound was added into culture medium at the following final concentration: nocodazole (0.3 μM), S-methyl DM1 (0.2 μM), demecolcine (1.6 μM) and vinblastine (1 μM). Average molecular number (ECFP-tubulin) and average residence time (inversely related to diffusion constant) were determined by fitting time autocorrelation curves (Fig. 4A) to a 3D diffusion model (see Methods).