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. 1994 Mar 1;91(5):1796–1800. doi: 10.1073/pnas.91.5.1796

Involvement of cardiovirus leader in host cell-restricted virus expression.

W P Kong 1, G D Ghadge 1, R P Roos 1
PMCID: PMC43250  PMID: 8127884

Abstract

Theiler murine encephalomyelitis virus designates a number of picornavirus strains that are classified into two subgroups on the basis of their different biological activities. DA strain and other members of the TO subgroup produce a chronic demyelinating disease in which the virus persists and manifests a restricted expression. Mutagenesis studies of the DA strain leader (L) coding region, which is located at the 5' end of the polyprotein coding region, demonstrate that L is completely dispensable for infection of some cells; in addition, nucleotides can be inserted into the L coding region with no loss in infectivity, indicating that Theiler murine encephalomyelitis virus may be used as a vector for delivering foreign sequences. In other cells, L is critical for plaque formation and efficient viral multiplication. These findings raise the possibility that L may play a role in the DA-induced demyelinating restricted infection. The functions of L, and even its presence within the genome, vary among picornaviruses, reflecting the various requirements for viral growth among different host cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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