FIG 7.

Levels of 3PGA, AMP, and CMP plus UMP in germinated cwlJ sleB B. megaterium spores incubated at 4 or 37°C. Spores of B. megaterium strain PS4241 (cwlJ sleB) were germinated with glucose, harvested, washed, and incubated at 4 or 37°C, and 3PGA, AMP, and CMP plus UMP were extracted and determined as described in Materials and Methods. At most, 8% of the initial dormant spore 3PGA and AMP was lost in germination. Values shown are averages from duplicate determinations in one experiment, and 3PGA, AMP, and CMP plus UMP values did not deviate by more than 12, 18, and 30%, respectively, from average values. The symbols used are as follows: circles, 3PGA; triangles, AMP; squares, CMP plus UMP; solid lines, incubation at 4°C; dashed lines, incubation at 37°C. NMR peaks corresponding to ADP or ATP were not detected in these samples, indicating that levels of these two nucleotides were not more than ∼5% of AMP levels in spores incubated for 3 to 8 days.