TABLE 2.
AMP and 3PGA levels in wild-type B. subtilis spores incubated for 2 days at various temperaturesa
| Incubation temp (°C) | Amt of molecule (μmol/g dry spores) |
|
|---|---|---|
| AMP | 3PGA | |
| 4 | 0.7 | 2.7 |
| 37 | 0.7 | 2.6 |
| 50 | 0.8 | 2.7 |
a
Spores of B. subtilis strain PS533 (wild type) were prepared on 2× SG plates at 37°C and incubated in water at various temperatures for 2 days. Spores were harvested and purified, and AMP and 3PGA were extracted, purified, and quantitated as described in Materials and Methods. Variations in values for AMP and 3PGA did not deviate by more than 25 and 15%, respectively.