FIG 1.
Sequence analysis of five Rpf homologues. (A) Multiple-sequence alignment of the Rpf domain from the five predicted Rpf proteins in S. coelicolor, together with RpfB from Mycobacterium tuberculosis (RpfBMTB). Identical residues are highlighted in black. Similar residues are indicated in gray background. Key catalytic (Glu) and substrate-binding residues (Ser/Thr and Trp) are indicated with asterisks. (B) Schematic representation of the functional domain organization of the predicted Rpf proteins, along with M. luteus Rpf and RpfBMTB. Sec, general secretion signal; Rpf, resuscitation-promoting factor domain; LysM, lysin motif; DUF348, domain of unknown function 348; G5, peptidoglycan-binding domain. Mtb, M. tuberculosis.