FIG 2.

Transcript levels of Rpf-encoding genes in S. coelicolor throughout growth and development. (A) The S. coelicolor wild-type strain M145 was grown at 30°C on MS agar medium and TSB-YEME liquid medium. At the times indicated, total RNA was extracted from cells, and transcripts bearing the rpfA, rpfC, and rpfD coding regions were quantified using RT-qPCRs. Transcript levels were normalized to total RNA mass and PCR product length. The quantification cycle value of each no-RT control was greater than that of the +RT samples by at least 10 cycles. Quantification cycle values of all no-template controls were consistently greater than 40 cycles. All data are presented as means ± standard errors (n = 1 to 3). au, arbitrary units. (B) rpf promoter activities were monitored in TSB-YEME liquid medium for 12 h postinoculation using a luminescence-based reporter. The inset panel (positive control) represents the activity of the strong constitutive ermE* promoter fused to the lux genes over the same 12-h time course. Data are presented as means ± standard errors (n = 8). RLU, relative light units.