FIG 1.
Swimming of strains expressing different cckA alleles. The swimming ability of different strains expressing different cckA alleles was tested. (A) Swimming of LC5 strain (WS8N ΔfleQ::Kanr ΔcckA::ΩSpc) expressing wild-type CckA or CckA L391F. AM1, positive control; LC5, negative control. (B) Swimming of strain CB1 (AM1 ΔfleQ::Kanr ΔcckA::ΩSpc) and complementation test with plasmids expressing CckAwt and CckA L391F. Plates were prepared with Sistrom's minimal medium containing 15 mM succinic acid and 0.22% agar. After inoculation, the plates were incubated anaerobically under continuous illumination for 60 h.