TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Rhodobacter sphaeroides strains | ||
| EA1 | AM1 derivative; ΔfleQ::Kanr cckAL391F ΔctrA::aadA | This work |
| EA2 | WS8N derivative; ΔctrA::aadA | This work |
| AM1 | WS8N derivative; ΔfleQ::Kanr cckAL391F | 43 |
| BV1 | WS8N derivative; ΔfleQ::Kanr cckAA387P | This work |
| BV2 | WS8N derivative; ΔfliF::aadA cckAF399C | This work |
| BV3 | AM1 derivative; ΔfleQ::Kanr cckAL391F ΔchpT::ΩSpc | This work |
| BV4 | WS8N derivative; ΔfleQ::Kanr ΔchpT::ΩSpc | This work |
| CB1 | AM1 derivative; ΔfleQ::Kanr ΔcckA::ΩSpc | This work |
| CB2 | WS8N derivative; ΔcckA::ΩSpc | This work |
| CD1 | WS8N derivative; ΔfleQ::Kanr ΔregB::ΩSpc | This work |
| CD2 | AM1 derivative; ΔfleQ::Kanr cckAL391F ΔregB::ΩSpc | This work |
| LC5 | WS8N derivative; ΔfleQ::Kanr ΔcckA::ΩSpc | This work |
| LC6 | AM1 derivative; ΔfleQ::Kanr ΔcckA::uidA-aad | This work |
| SP13 | WS8N derivative; ΔfleQ::Kanr | 22 |
| SP20 | WS8N derivative; ΔfliF::aadA | Laboratory collection |
| WS8N | Wild-type strain; spontaneous Nalr | 24 |
| Escherichia coli strains | ||
| LMG194 | Protein expression strain | Invitrogen |
| S17 | recA endA thi hsdR RP4-2-Tc::Mu::Tn7; Tpr Smr | 30 |
| TOP10 | Cloning strain | Invitrogen |
| Plasmids | ||
| pBAD/His | Expression vector of His6-tagged proteins; Apr | Invitrogen |
| pBAD/His-cCckA | pBAD/HisB expressing His6-cCckA | This work |
| pBAD/His-cCckA A387P | pBAD/HisB expressing His6-cCckA A387P | This work |
| pBAD/His-cCckA L391F | pBAD/HisB expressing His6-cCckA L391F | This work |
| pBAD/His-cCckA F399C | pBAD/HisB expressing His6-cCckA F399C | This work |
| pBBMCS53 | Transcriptional uidA fusion vector; Gmr | 34 |
| pBBMCS53_fliL2 | pBBMCS53 carrying the fliL2 promoter | This work |
| pCR2.1-TOPO | Cloning vector; Apr Kanr | Invitrogen |
| pJQ_ΔctrA::aadA | pJQ200 mp18 carrying ΔctrA::aadA | This work |
| pJQ_ΔregB::ΩSpc | pJQ200 mp18 carrying ΔregB::ΩSpc | This work |
| pJQ200_ΔcckA::ΩSpc | pJQ200 mp18 carrying ΔcckA::ΩSpc | This work |
| pJQ200_ΔchpT::ΩSpc | pJQ200 mp18 carrying ΔchpT::ΩSpc | This work |
| pJQ200mp18 | Mobilizable suicide vector for R. sphaeroides; Gmr | 27 |
| pPIRL | Vector that encodes tRNAs for rare codons; Cmr | 35 |
| pRK_cckA | pRK415 derivative expressing cckA | This work |
| pRK_cckAA387P | pRK415 derivative expressing cckAA387P | This work |
| pRK__cckAL391F | pRK415 derivative expressing cckAL391F | This work |
| pRK_cckAF399C | pRK415 derivative expressing cckAF399C | This work |
| pRK_chpT | pRK415 derivative expressing chpT | This work |
| pRK_ctrA | pRK415 derivative expressing ctrA | This work |
| pRK_regBL267 | pRK415 derivative expressing regBL267 | This work |
| pRK_regBS267 | pRK415 derivative expressing regBS267 | This work |
| pRK415 | pRK404 derivative used for expression in R. sphaeroides | 33 |
| pTOPO_cckA | pCR2.1 TOPO carrying cckA | This work |
| pTOPO_ctrAup-down | pCR2.1 TOPO carrying ctrAupdown | This work |
| pTOPO_ΔcckA::ΩSpc | pCR2.1 TOPO carrying ΔcckA::ΩSpc | This work |
| pTOPO_ΔcckA | pCR2.1 TOPO carrying ΔcckA | This work |
| pTZ_chpTup-down | pTZ19R carrying chpTupdown | This work |
| pTZ_ctrAupdown | pTZ19R BamHI− carrying ctrAupdown | This work |
| pTZ_ΔchpT::ΩSpc | pTZ19R carrying ΔchpT::ΩSpc | This work |
| pTZ_ΔctrA::aadA | pTZ19R BamHI− carrying ΔctrA::aadA | This work |
| pTZ_ΔregB | pTZ19R carrying ΔregB | This work |
| pTZ_ΔregB::ΩSpc | pTZ19R carrying ΔregB::ΩSpc | This work |
| pTZ_regBL267 | pTZ19R carrying regBL267 | This work |
| pTZ_regBS267 | pTZ19R carrying regBS267 | This work |
| pTZ19R | Cloning vector | Pharmacia |
| pTZ19R BamHI− | pTZ19R without BamHI site | Laboratory collection |
| pWM5 | Vector source of the uidA-aadA cassette | 28 |