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Cj1386 is a hemin-binding protein. (A) Purified Cj1386WT and Cj1386Y57A protein solutions at 50 μM. Cj1386WT has a yellowish hue, in contrast to Cj1386Y57A, which is colorless. (B) Cj1386 purification after size exclusion chromatography. Five microliters of purified protein was loaded and separated on a 14% denaturing SDS-PAGE gel. (C) Absorption spectrum of 10 μM purified Cj1386 in 100 mM NaCl, 20 mM Tris, pH 7.4, at 22°C.
