FIG 6.

Cj1386 and KatA proteins interact. (A) Immunoprecipitation of KatA from cross-linked ΔperR protein lysate using an anti-KatA antibody. (B) Immunoprecipitation of Cj1386 from cross-linked ΔperR protein lysate using an anti-Cj1386 antibody. (A and B) Immunoprecipitated protein samples were separated by SDS-PAGE followed by immunoblotting with either anti-KatA or anti-Cj1386 antibodies to visualize protein interactions. Immunoprecipitation using an anti-Fur antibody was performed as a negative control. Purified Cj1386 and KatA proteins were run as positive controls. IP, immunoprecipitation; WB, Western blot; Input, whole-cell lysate from the ΔperR mutant.