TABLE 1.
Bacterial strains used in this study
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | endA1 hsdR17 (rK− mK−) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR[ϕ80dlacΔ(lacZΔM15)] | Invitrogen |
| BL21(DE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3) | Novagen |
| Rosetta | F− ompT hsdSB(rB− mB−) gal dcm pRARE Camr | Novagen |
| AS1082 | BL21(DE3)(pGST+katA) Ampr | Flint et al. (7) |
| AS1091 | Rosetta(pGST+cj1386) Ampr Camr | This study |
| AS1138 | Rosetta(pGST+cj1386Y57A) Ampr Camr | This study |
| AS1142 | Rosetta(pGST+cj1386H46A) Ampr Camr | This study |
| AS1150 | Rosetta(pGST+cj1386C89A) Ampr Camr | This study |
| C. jejuni strains | ||
| AS144 | NCTC 11168 | National Collection of Type Cultures |
| AS216 | AS144 ΔperR::Camr | Palyada et al. (38) |
| AS433 | AS144 ΔkatA::Camr | Palyada et al. (38) |
| AS942 | AS144 Δcj1386::Camr | Flint et al. (7) |
| AS1028 | AS942 cj1386::Camr Kanr | Flint et al. (7) |
| AS1139 | AS942 cj1386Y57A | This study |
| AS1144 | AS942 cj1386H46A | This study |
| AS1149 | AS942 cj1386C89A | This study |
| Plasmids | ||
| pRY111 | Camr resistance gene | Yao et al. (39) |
| pRRK | Cloning vector used for complementation of mutants, Kanr | Reid et al. (40) |
| pUC19 | Cloning vector, Ampr | New England Biolabs |
| pGST | Protein expression vector with GST tag and IPTG inducible promoter | Sheffield et al. (8) |
a
Camr, chloramphenicol resistance gene; Kanr, kanamycin resistance gene; Ampr, ampicillin resistance gene.