TABLE 4.

Hemin quantification of KatA immunoprecipitated from C. jejuni NCTC11168 and Δcj1386+cj1386^Y57A strains^a

Source of immunoprecipitated KatA	Concn of KatA subunits (nM)	Concn of hemin (nM)	Hemin/KatA ratio	Source or reference
C. jejuni NCTC11168	7.4	6.99 ± 0.19	0.94	7
Δcj1386 strain	7.4	0.19 ± 0.10*	0.03	7
Δcj1386+cj1386WT strain	7.4	6.41 ± 0.54	0.87	7
Δcj1386+cj1386Y57A strain	7.4	0.29 ± 0.18*	0.04	This study

^aThe amount of hemin is represented as the mean concentration of hemin detected ± standard errors (in nM) for equal starting concentrations of KatA protein for each strain tested. Experiments were repeated in biological triplicate. Student's t test was used to determine statistical significance, with P values of <0.05 (*) considered significant.