FIG 3.
TraR activates transcription from P3rpoH in vitro. (A) Transcripts from a supercoiled plasmid template containing the P3rpoH promoter produced at a range of concentrations of purified wild-type TraR or mutant TraR D6N and resolved on 6% acrylamide-7 M urea gels. (B) Quantification of triplicate determinations of transcripts produced with 500 μM wild-type or D6N mutant TraR as described for panel A, normalized to transcripts produced in the absence of TraR. (C) Transcripts from P3rpoH produced in the absence of factors or in the presence of 1 μM wild-type TraR with or without the indicated concentrations of ppGpp. (D) Quantification of transcripts as described for panel C, normalized to transcription in the absence of factors (1.0).