TABLE 1.
Strains selected for sequencing and their phenotypes
| Strain | Colony | Chainingb | % motility (±SD)c | % plaque area (±SD)d |
|---|---|---|---|---|
| R9 | Rough | +++ | 29 (±3) | 41 (±9) |
| R23 | Smooth | + | 55 (±13) | 0 |
| R35 | Smooth | ++ | 102 (±26) | 0 |
| R57 | Smooth | + | 112 (±15) | 87 (±13) |
| R60.1 | Smooth | ++ | 87 (±23) | 21 (±6) |
| ΔsecA2 straina | Rough | ++++ | 9 (±2) | 29 (±9) |
a
The parent ΔsecA2 strain was included as a reference strain.
b
Chaining score interpretation by phase microscopy. +, single cells and chains of up to 3 cells/field at ×40 magnification; ++, single cells and chains of up to 4 cells/field at ×40 magnification; +++, chains of up to 6 cells/field at ×40 magnification; ++++, chains of >6 cells/field at ×40 magnification.
c
Expressed as an average percentage (standard deviation) of the swarming area normalized to that of the WT strain on 0.4% LB agar after 48h at 30°C.
d
Expressed as an average percentage (standard deviation) of the plaque area compared to that of the WT strain.