TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| CF7789 | MG1655 ΔlacIZ (MluI) | Michael Cashel |
| TR1-5CF7789 | CF7789 csrA::Kanr | 25 |
| KSB837 | CF7789 ϕ(csrB-lacZ) | 26 |
| IFC5010 | KSB837 csrA::Kanr | This work |
| BAKSB837 | KSB837 barA::Kanr | 25 |
| UYKSB837 | KSB837 uvrY::Camr | 25 |
| ECL5336 | MC4100 ackA::Tetr::pta | 27 |
| IFC5011 | CF7789 ϕ(uvrY-lacZ) operon fusion | This work |
| IFC5012 | TR1-5CF7789 ϕ(uvrY-lacZ) operon fusion | This work |
| IFC5013 | CF7789 ϕ(PlacUV5-uvrY22′-′lacZ) leader fusion | This work |
| IFC5014 | TR1-5CF7789 ϕ(PlacUV5-uvrY22′-′lacZ) leader fusion | This work |
| IFC5015 | KSB837 ackA::tetR::pta csrA::Kanr | This work |
| IFC5016 | KSB837 uvrY::Camr csrA::Kanr | This work |
| IFC5017 | KSB837 barA::Camr csrA::Kanr | This work |
| Plasmids | ||
| pACT3 | Low-copy-no. vector, Camr | 31 |
| pEXT21 | Low-copy-no. vector, Spr | 31 |
| pBA29 | barA in blunt-ended VspI site of pBR322, Tetr | 25 |
| pAH125 | CRIM vector for transcriptional lacZ fusions, Kanr | 29 |
| pINT-ts | CRIM integration vector, Ampr | 29 |
| pUV5 | CRIM vector for translational fusions, Ampr | 30 |
| pAH125-bla | CRIM vector for transcriptional lacZ fusions, Ampr | This work |
| pINT-cat | CRIM integration vector, Camr | This work |
| pMX539 | uvrY in SmaI site of pACT3, Camr | This work |
| pMX540 | uvrY D54Q in SmaI site of pACT3, Camr | This work |
| pMX541 | uvrY in blunt-ended NdeI site of pBA29, Tetr | This work |
| pMX542 | barA promoter in pEXT21, Spr | This work |
| pMX543 | uvrY under the control of barA promoter in pEXT21, Spr | This work |
| pMX544 | csrA in HindIII and PstI sites of pEXT21, Spr | This work |
| pMX545 | csrB in pGEMT-Easy, Ampr | This work |
| pAH-uvrY | uvrY-lacZ operon fusion, Ampr | This work |
| pUV-uvrY22 | uvrY-lacZ leader (plus 22 codons) fusion under the control of the lacUV5 promoter Ampr | This work |