FIG 3.

Loss of M. buryatense 5GB1 native plasmid results in more genetically tractable variants capable of being conjugated with small IncP-based plasmids. (A) Selection scheme used to obtain the more genetically tractable variant strain 5GB1S. This selection was repeated twice. (B) Number of transconjugants containing the small IncP-based plasmid pCM66 for M. buryatense 5GB1, selected strain 5GB1S, and a strain intentionally cured of the native plasmid, 5GB1C. N.D., not detected. (C) Genome sequence reads mapped to chromosome versus native plasmid for strains 5GB1 and 5GB1S. RPKM, reads per kilobase of gene per millions of reads in the sample.