FIG 3.

Changes of CODH gene expression at the transcriptional and translational levels after CO addition. (A) mRNA levels of the CODH gene (TON_1018) in the wild-type, ΔCorQ, ΔCorR, ΔCorQ/corQR^↑, and ΔCorR/corQR^↑ strains. The mRNA quantity was measured by RT-qPCR. The y axis indicates the relative fold difference when the value for the wild-type strain in the absence of CO was set equal to 1.0. (B) Protein levels of CODH in the wild-type, ΔCorQ, ΔCorR, ΔCorQ/corQR^↑, and ΔCorR/corQR^↑ strains. The amount of the CODH protein was monitored by Western blot analysis. WT, wild-type strain; −, no injection of CO (gray bars); +, injection of CO (black bars).