FIG 4.

Strain CIP82110 as a representative of nonmotile strains forming flat biofilms. (A and B) IMARIS isosurface representation (A) and section view (B) of the CLSM images of the biofilms stained with Syto 9 and propidium iodide. The scale bars represent 20 μm. (C) Twenty-four-hour swimming plate (TSB plus 0.25% agar) of the nonmotile strain CIP82110 compared to the swimming phenotype of strain H25, represented by the dashed circle. (D) SEM observation of the biofilm formed, at 2 × 10⁴ magnification.