FIG 3.

Reserve carbohydrate of a mixture of rumen protozoa and bacteria after a pulse dose of glucose at 20 min. (A and C) Five millimolar glucose; (E and G) 4.62 mM glucose; (B, D, F, and H) 20 mM glucose. Shown are separate values for bacteria and protozoa for cows 491 (A and B), 472 (C and D), 478 (E and F), and 492 (G and H). Mixtures of protozoa and bacteria were prepared and harvested according to the flowchart shown in Fig. 1. Reserve carbohydrate is expressed as mM monomeric glucose equivalents to be in same units as glucose in the medium. Each panel represents data from one experiment, and each datum point represents one sample replicated in triplicate.