FIG 5.

Scheme of the experimental procedure. (A) D. radiodurans R1 (ATCC 13939) cells were cultured in TGY medium to exponential (OD₆₀₀ = 1) or stationary (OD₆₀₀ = 3) phase. Cells were then irradiated while cold and then recovered in fresh medium for 120 min. RNA and protein total lysates were prepared for analysis. RT, room temperature. (B) Western blotting for RecA expression under 15 kGy of ionizing radiation. (C) ELISA (1 μg of RNA was used for each test) quantifying the oxidation damage of the DNA in irradiated samples and the control. 8OG, 8-oxoguanine.