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Upregulation of GND1 by pho13Δ requires the transcription factor Stb5 and the Stb5-binding site of GND1. RT-qPCR was used to measure the expression of GND1 in strains with pho13Δ or stb5Δ. The pho13Δ GND1.1 mutant had an SNP (CTGTGTTA, where the SNP is underlined) in the Stb5-binding sequence (CGGTGTTA) upstream of the GND1 coding region.
