TABLE 3.

gRNA (gBlocks) sequences (388 bp)

Parts	Sequence	Length (bp)
SNR52 promoter	TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC	269
GND1.1 gRNA (target specific)	GGTGTTACATTACGGTGCCT	20
Structural crRNA	GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGGTGC	79
SUP4 terminator	TTTTTTTGTTTTTTATGTCT	20