FIG 3.

Time course of fluorescence development. (A) C. difficile exconjugants producing mCherryOpt from pDSW1728 were induced with 400 ng of aTet per ml, fixed, removed from the anaerobic chamber, and photographed at the times indicated. “Prior” refers to an unfixed sample photographed immediately after removal from the anaerobic chamber. (B) C. difficile/pDSW1728 (mCherryOpt) or C. difficile/pRPF185 (Neg) were grown and induced as described above. Cells were fixed or not as indicated, transferred to PBS, and removed from the anaerobic chamber. Fluorescence (red filter) was recorded using a plate reader at 15-min intervals and normalized to the OD₆₀₀ at time zero. The data points represent the means and standard deviations of three independent cultures per group, all grown and processed on the same day. These results are representative of three experiments.