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. 2015 Feb 10;81(5):1735–1743. doi: 10.1128/AEM.03214-14

TABLE 1.

Primers and probes used for detection of functional genes in E. coli isolates

Gene Function Primer/probe name Sequence Size (bp)
sfmH Fimbrial-like adhesion protein sfmH.F 5′-TGCGTCTGGAAGCCAGTGCC-3′ 220
sfmH.R 5′-GCGCTAAACGGCCCYTCGGT-3′
sfmH.P 5′-GTCTGGATGCAGCTGCGGCAG-3′
rfaI Lipopolysaccharide biosynthesis protein rfaI.F 5′-TGCTTGGCGCAATAACGAGCA-3′ 142
rfaI.R 5′-TCGGCCCGACAAAACCCTGG-3′
rfaI.P 5′-TGCGCRCTTGGATAACCGGCCCAGTA-3′
fucK Sugar kinases for 5-C and 6-C sugars fucK.F 5′-ACAGAACAGCGCCGCAGCAA-3′ 162
fucK.R 5′-ACCTGGGCGCTRGGACCAT-3′
fucK.P 5′-CGCCCTTTTTGGCGCTGGTGCCG-3′