FIG 1.

Experimental setup for studying the mode of action of HCV inactivation procedures. (A) Chemical disinfectants and virus were mixed at a ratio of 10 to 1, and the mixture was incubated at room temperature for 1 min before the infectivity was determined by TCID₅₀ assay. In the case of heat treatment or UV irradiation, the virus were mixed at a ratio of 10 to 1 with DMEM and heated at 80°C for 5 min or UV irradiated before determination of the TCID₅₀. (B) Virus particle-associated RNA was extracted and measured by qRT-PCR. Purified RNA was used to transfect naive Huh7.5 cells by electroporation. (C) After 72 h, Huh7.5 cells were lysed, and HCV RNA was analyzed by qRT-PCR. (D) The supernatant of the cells was harvested and used to infect naive Huh7.5 cells to determine virus titers.