FIG 2.

Influence of treatment procedures on HCV infectivity and RNA integrity. (A) Chemical disinfectants, heat, and UV treatment were tested in a quantitative suspension assay for their efficiency in inactivating HCV by determination of the TCID₅₀. (B) HCV RNA of the respective supernatant was isolated and quantified by RT-PCR. (C) The isolated RNA was used for re-electroporation of Huh7.5 cells. After 72 h, RNA was extracted and quantified by qRT-PCR. (D) Limiting-dilution assay was used to determine the TCID₅₀ of the viral supernatants. Depicted are the means plus the standard deviations of three independent experiments. The background level of the assay is shown as a dotted line. Statistical analysis was performed using a one-tailed Student t test.