FIG 4.
Biosynthetic scheme for exclusive production of pneumocandin B0. (A) Pneumocandins from the wt strain. The thinner arrow indicates the minor route for pneumocandin B0 biosynthesis, while the thick arrow indicates the predominant route for pneumocandin A0 biosynthesis. (B) Blockage of pneumocandin A0 biosynthesis in the GLOXY4 disruption mutant leads to exclusively pneumocandin B0 production. The hydroxylated proline residues originating from l-proline are shown in blue, and the hydroxylated 4-methyl-l-proline residue originating from l-leucine is shown in red.