TABLE 1.

GLOXY4 primers used in this study^a

Primer name	Sequence (5′ to 3′)	Restriction enzyme	PCR fragment size (kb)
S1	CAGCTGGAGAAGTTCAAGAGCAGGAT	PvuII	1.2
R1	GGGCCCCAGGGCAGATATTTCAATTAG	ApaI
S2	GGCGCGCCGATGGTCCAAAACCTCAAAG	AscI	1.2
R2	CCTGCAGGGTTGGGTTGGAGTACAAAAG	SbfI
W	GGAGGCTTTCATCGTCGTC		1.5 (wt)
X	CGAGTCCTCCCACATCAT		3.4 (KO)
Y	TGACGGACGGCTTACATG		No product (wt)
I	CGAGGGCAAAGGAATAGAGTAG		2.7 (KO)

^aKO, knockout. Restriction enzymes were used for the construction of disruption vectors. Underlined sequences identify the restriction sites.