Figure 3.

Endothelial Cells Promote PP Proliferation by Secretion of EGFL7

(A) Cell number per mm² 4 days after HUES8-derived PPs were cocultured with endothelial cells or treated with CM (n = 3).

(B) Transcriptome analysis of gene expression in BJ cells and AKT-HUVECs by RNA-seq. The blue line indicates the cutoff line of a 30-fold increase in gene expression in AKT-HUVECs as compared with BJ cells. The red dot indicates EGFL7.

(C) Number of PDX1⁺ cells per mm² 4 days after hESC-derived PPs were treated with different recombinant proteins (300 ng/ml) (n = 3).

(D) PDX1⁺ (left) or PDX1⁺/Ki67⁺ cell number per mm² (right) 4 days after HUES8-derived PPs were treated with CM or 300 ng/ml EGFL7 (n = 3).

(E) Representative images of (D).

(F) PDX1⁺/Ki67⁺ cell number per mm² 4 days after HUES8-derived PPs were cocultured with AKT-HUVECs carrying shRNA targeting EGFL7 (shRNA-EGFL7) or AKT-HUVECs carrying scrambled control shRNA (scrambled) (n = 3).

(G) Representative images of (F).

Data were presented as mean ± SD.