Table 1. Sequences, annealing temperatures (Tan.) and predicted product sizes of the primers used.
Gene NCBI accession number (rat and human) | Updream primer | Downdream primer | Tm (°C) | Size (bp) |
BMP-3 | ||||
NM_017105.1 | TTGGCTGGAGCGAATGGATTA | GCTCAGGAATCCCAGAGACGAC | 56 | 159 |
NM_001201.2 | ||||
BMP-4 | ||||
NM_017105.1 | TTTGTTCAAGATTGGCTCCCAAG | AAACGACCATCAGCATTCGGTTA | 55 | 101 |
NM_001201.2 | ||||
BMP-5 | ||||
NM_012827.2 | TCCACAGAACAATTTGGGCTTACA | ACCATGAACGGCTGCTTTGAC | 55 | 120 |
BC020546.2 | ||||
BMP-6 | ||||
NM_013107.1 | CGCCTTCCTCAACGACCGCGG | GGAATCTGGGATAAGTTGAA | 55 | 120 |
NM_001718.4 | ||||
BMP-7 | ||||
NM_001191856.1 | TCCGGTTTGATCTTTCCAAGA | CCCGGATGTAGTCCTTATAGATCCT | 55 | 81 |
NM_001719.2 | ||||
BMPR-IA | ||||
S75359.1 | GCCGTTTTGAAGCTGATGTCA | TCTTTGCGAGCGTCTTCTTGA | 55 | 88 |
NM_004329.2 | ||||
BMPR-IB | ||||
NM_001024259.1 | TGGCTGACATGTACAGCTTTGG | GGCACTCGTCACTGCTCCAT | 56 | 198 |
D89675.1 | ||||
BMPR-ll | ||||
NM_080407.1 | ACTGCAGATGGACGCATGG | AATCTCGATGGGAAATTGCAG | 55 | 199 |
NM_001204.6 | ||||
β-actin | ||||
NM_031144.2 | GGCACCACACTTTCTACAATG | GGGGTGTTGAAGGTCTCAAAC | 55 | 133 |
NM_001101.3 |