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. 2015 Jan 15;4(2):239–254. doi: 10.1016/j.stemcr.2014.12.007

Figure 2.

Figure 2

BMP2 Is Produced by Stromal and Endothelial Cells in Luminal Tumors

(A) qPCR analysis of BMP2 and BMP4 expression in indicated cellular fractions from normal human mammoplasty and adipose tissue samples. Data represent the mean ratio to reference ± SEM, n = 5 independent experiments.

(B, C, and E) IHC staining for BMP2, epithelial (Pan Keratin), SMA, endothelial (CD31), and pan leukocyte (CD45-pan Leu) of serial sections of the same luminal tumor. (B) Normal peritumoral tissue. (C and E) Tumor tissue. Arrows indicate the following cells: myoepithelial/basal (Myo/Bas), luminal epithelial (Lu), tumor cells (Tum), leukocytes (Leuco), stromal/fibroblast (Stro), or endothelial (Ve). Scale bar represents 20 μm.

(D) Quantification of BMP2 IHC staining of 350 luminal and 30 basal tumors. Data are presented as a percentage of BMP2-positive vessels or stromal cells for each subtype.