Figure 4. Effects of girdling on the expression of genes associated with (A) Glycolysis, TCA cycle, and mitochondrial electron transport pathways and (B) flavonoid biosynthetic pathway.

The values in red and green indicate log₂ fold increases and decreases, respectively, in the expression of specific genes in partial girdled (PG) and fully girdled (FG) leaves compared to control leaves. Experimental conditions 1–6 (left to right) are leaves from 120-day-old plants that were developing storage roots: (1) 0 h PG leaves, (2) 0 h FG leaves, (3) 2 h PG leaves, (4) 2 h FG leaves, (5) 4 h PG leaves, and (6) 4 h FG leaves; conditions 7–10 (left to right) are leaves from 45-day-old seedlings that have no storage roots developed: (7) 0 h PG leaves, (8) 0 h FG leaves, (9) 2 h PG leaves, and (10) 2 h FG leaves.