Figure 5. Role of the RcsAAB box on regulation of expression of HmsD by Rcs in Y. pestis.

Western blots of total protein-matched lysates prepared from cells with mutation of (a) RcsXXX, (b) RcsAXX, (c) RcsABX or (d) RcsXAB were analyzed by anti-Myc antibody. Levels of HmsD were quantitated by using ImageJ from at least two independent experiments: numbers below blots indicate the ratio of HmsD from the indicated strain compared to that from wild-type hmsD-Myc₂ strain (WT). Strain designations (Supplementary Table S1) are: 1, KIM6+; 2, hmsD-Myc₂; 3, Rcs box mutation, hmsD-Myc₂; 4, functional rcsA, Rcs box mutation, hmsD-Myc₂; 5, ΔrcsB, Rcs box mutation, hmsD-Myc₂; 6, ΔrcsB, Rcs box mutation, hmsD-Myc₂/pUC19; 7, ΔrcsB, Rcs box mutation, hmsD-Myc₂/pYC332. pYC332: p-rcsB. Full-length blots are presented in Supplementary Fig. S6–S9 online.