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. 2015 Feb 12;5:8412. doi: 10.1038/srep08412

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Electrophoretic mobility shift assays (EMSA) of hmsC promoter DNA incubated with increasing concentrations of RcsB. hmsD promoters with wild-type RcsAAB box (a) or with the mutated RcsAAB box RcsXXX (b), RcsAXX (c), RcsABX (d), or RcsXAB (e) were tested with identical protein combinations. Lane 1, hmsD probe alone; lanes 2–10, 100 ng hmsCDE probe with 400, 800, 1200, 1600, 2000, 2400, 4000, 6000 or 8000 ng of RcsB in the 16 μL reaction.