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. 2015 Feb 15;142(4):774–786. doi: 10.1242/dev.112532

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — UXT is essential for zebrafish angiogenesis. (A-C) RT-PCR verification of angiogenesis markers in 18 hpf, 24 hpf or 30 hpf zebrafish embryos injected with 4 ng of control morpholino (Ctrl MO) or with 4 ng of UXT morpholino (UXT MO). Data shown are the mean±s.e.m. (at least three independent experiments); *P<0.05, **P<0.01 versus the corresponding control. (D) Whole-mount in situ hybridization of embryos injected with 4 ng of control morpholino (Ctrl MO) or 4 ng of UXT morpholino (UXT MO). Riboprobes against the angiogenesis marker kdrl, the pronephric marker pax2.1 and the erythroid marker gata1 were visualized at 22 hpf. The red arrowhead indicates the decreased expression of kdrl in UXT-deficient embryos.