Fig. 4. : detection of Wuchereria bancrofti DNA in samples of serum and urine from patients using polymerase chain reaction (PCR). A: internal PCR. Line 1: molecular weight marker of 100 bp; 2-15: serum samples of patients; 16: positive control; 17: negative control; B: internal PCR (WbF/Wb2). 1: molecular weight marker of 100 bp; 2-15: urine samples from patients; 16: positive control; 17: negative control; C: internal PCR. 1: molecular weight marker of 100 bp; 2-7: urine samples from patients; 8: negative control; 9: positive control; 10-15: blood samples from patients; 16: positive control; 17: negative control; D: semi-nested PCR. 1: molecular weight marker of 100 bp; 2-15: urine samples from patients; 16: positive control; 17: negative control; E: semi-nested PCR. 1: molecular weight marker of 100 bp; 2-7: urine samples from patients; 8: positive control; 9: negative control.