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. 2014 Dec 17;89(5):2575–2589. doi: 10.1128/JVI.02791-14

FIG 5.

FIG 5

PKR antibodies variably report PKR in G3BP1-induced SGs. (A) G3BP1 was expressed from either the pcDNA 3.1 HisC-G3BP1 (SC) construct or pG3BP1-GFP (PSc and BD), and PKR was stained with three different antibodies (SC, PSc, and BD, as indicated) in either HeLa or G3BP1 KO cells as represented in the indicated colors. The antibody epitopes on PKR are as follows: BD, second RNA-binding domain; SC, kinase domain; PSc, N-terminal RNA-binding domain. The BD antibody does not recognize mouse PKR protein. The insets represent the boxed areas. (B) Antibody specificities for the PSc and BD antibodies were demonstrated in IFA after control or PKR-specific siRNAs were used to deplete PKR signal using IF in HeLa cells. (C) Western blot analysis was performed on HeLa cells treated with either control siRNA or PKR-specific siRNA.