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TLR4 internalization. Internalization of TLR4 was measured by receptor removal from the cell surface. MoDCs were stimulated with LPS (A), HAd5 with or without FX (B), and HAd5 with or without FX plus IgG (C) or LPS as a positive control for 3 h and subsequently stained for TLR4. This experiment was performed using 3 individual donors with similar results. NT, nontreated; NS, nonstained; LPS, lipopolysaccharide; IC, immune-complexed adenovirus; Ad, adenovirus; FX, coagulation factor X.
