FIG 4.

ELISA titration of mouse antisera after immunization with EGFP-derived chimeric assemblies. (A) SDS-PAGE of antigens used for mouse immunization: EGFP-HT-VP2-466 VLP (lane 1), HT-VP2-466 VLP (lane 2), and bacterial GFP (lane 3). The table shows the antigen dose administered per mouse (μg/dose; three injections). (B, C) ELISA titration of mouse antisera in plates coated with IBDV-derived VLPs from rVV vT7lacOI-POLY (B) or purified bacterial HT-EGFP (C). Mice were immunized with GFP-CAP (blue), CAP (green), CAP-GFP (red), and GFP (purple), all with adjuvant. (D, E) ELISA titration of mouse antisera after immunization with the same antigens described for panels B and C without adjuvant; plates were coated as described for panels B and C. Dashed lines, background level measured for an unrelated mouse serum sample.