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Incorporation of EGFP-HT-VP2-466 into chimeric capsidsa
| Coinfection ratiob | % EGFP-HT-VP2-466 incorporated into capsids | Mean copy no. of EGFP-HT-VP2-466b |
|---|---|---|
| 1:1 | 18 | 148 |
| 3:1 | 27 ± 4 | 210 ± 31 |
| 5:1 | 31 ± 2 | 240 ± 16 |
| 10:1 | 25 | 195 |
The percentage of capsids incorporating EGFP-HT-VP2-466 and the mean copy number of EGFP-HT-VP2-466 were estimated from Coomassie blue-stained gels of fractions 5 to 7, assuming that all assemblies are T=13 capsids.
Ratio of rBV/EGFP-HT-VP2-466 to rBV/HT-VP2-466.
