FIG 6.

Role of E2F1 in regulation of Cdk1 in E6-expressing cells. (A and B) Total protein extracts from NIKS (A) and RPE1 (B) cells expressing vector, E6, and F2V were analyzed for E2F1 by Western blotting. β-Tubulin was used as a loading control. (C) RPE1-F2V cells were transfected with siRNA specific to E2F1 for 36 h, and the levels of E2F1 and Cdk1 were measured by Western blotting. β-Tubulin was used as a loading control. Bottom, quantification of the relative protein levels in the experiments whose results are shown in panels A to C.