TABLE 6.
Seal11 NA kinetic analysis and drug susceptibility assessment
| Virus name | Subtype | Parameter value |
||||
|---|---|---|---|---|---|---|
| kcat (s−1) ± SD | Km (μM) ± SD | kcat/Km (M−1 s−1) | IC50 (nM) ± SDa |
|||
| Oseltamivir carboxylate | Zanamivir | |||||
| RecNA | ||||||
| A/harbor seal/Massachusetts/1/2011 | N8 | 328,281 ± 5,769 | 3.227 ± 0.205 | 1.02 × 1011 | 1.42 ± 0.12 | 2.14 ± 0.70 |
| A/Perth/16/2009 | N2 | 32,039 ± 501 | 7.985 ± 0.365 | 4.01 × 1010 | 0.30 ± 0.07 | 0.44 ± 0.10 |
| Virus | ||||||
| A/harbor seal/New Hampshire/179629/2011 | A(H3N8) | NDb | ND | 0.66 ± 0.14 | 1.29 ± 0.39 | |
| Reference virus | ||||||
| A/California/12/2012 | A(H1N1)pdm09 | ND | ND | 0.28 ± 0.02 | 0.13 ± 0.02 | |
| A/Texas/23/2012c | A(H1N1)pdm09 | ND | ND | 160.82 ± 18.59 | 0.16 ± 0.02 | |
a
Average value from at least three measurements.
b
ND, not determined.
c
Oseltamivir resistant with His275Tyr substitution in the NA.