FIG 5.

Effects of cell cycle and peroxisomal division mutant yeasts on TBSV RNA recombination. (Top) Replication of the TBSV DI-AU-FP repRNA in the shown yeast strains coexpressing the tombusvirus p33 and p92 replication proteins was measured by Northern blotting 24 h after the launch of TBSV replication. The ratio of recRNAs and degRNAs was calculated as for Fig. 1. (Middle) The accumulation level of repRNA was normalized based on the rRNA. (Bottom) The accumulation levels of His₆-p92 and His₆-p33 were tested by Western blotting. The samples were obtained from independent yeast colonies. The experiments were repeated three times. The asterisk indicates detergent-resistant p33 homodimers.