FIG 2.

Acetylation modifications of α-tubulin and histone 3. (A) 293T cells and HDAC6-overexpressing 293T cells were treated with purified rCap for 6 h, TSA for 3 h, NOC for 3 h, or NaBut for 6 h, and immunoblots of cell lysates was probed with mouse anti-acetylated α-tubulin MAb and rabbit anti-acetylated histone 3 antibody to analyze the level of acetylated α-tubulin and histone 3. Data are represented as means ± standard deviations (SD) (n = 3; one asterisk [*] represents P < 0.05, and two asterisks [**] represent P < 0.01) Ac, acetylated. (B) PK15 cells were infected with PCV2 for 12, 24, 48, and 72 h. Cell lysates were probed with anti-Cap, anti-acetylated histone 3, and anti-histone 3 antibodies in immunoblotting experiments.