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. 2014 Dec 10;89(5):2530–2542. doi: 10.1128/JVI.03506-14

FIG 11.

FIG 11

Effect of NgK and MgK on induced reactivation in latently infected mice. C57BL/6 mice were infected ocularly with 2 × 105 PFU of NgK, MgK, or RgK virus/eye, while some mice were infected with 2 × 106 PFU of NgK or MgK virus/eye. On day 28 p.i., TG from infected mice was isolated, and each individual TG was incubated in 1.5 ml of tissue culture medium at 37°C. A 100-μl aliquot was removed from each culture daily for 15 days and used to infect RS cell monolayers. The RS cells were monitored daily for the appearance of CPE for 5 days to determine the time of first appearance of reactivated virus from each TG. The results are plotted as the number of TG that reactivated daily. Numbers indicate the average time that the TG from each group first showed CPE ± the SEM. Each point represents the mean ± the SEM from 6 (NgK, 2 × 105 PFU/eye), 5 (MgK, 2 × 105 PFU/eye), 8 (RgK, 2 × 105 PFU/eye), 8 (NgK, 2 × 106 PFU/eye), and 8 (MgK, 2 × 106 PFU/eye). At 2 × 105 PFU/eye, 5 of 12 TG in NgK-infected mice, 7 of 12 TG in MgK-infected mice, and 2 of 10 TG in RgK-infected mice did not reactivate, whereas all 8 TG infected with 2 × 106 PFU of NgK or MgK/eye were reactivated.