TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference(s) |
|---|---|---|
| Strains | ||
| E. coli | ||
| α-select competent cells | F− deoR endA1 recA1 relA1 gyrA96 hsdR17(rk− mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 ϕ80lacZΔM15 λ− | Bioline, United Kingdom |
| CA434 | HB101 [F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(Smr) glnV44 λ−] with plasmid R702 | 16, 17 |
| C. difficile | ||
| 630 (wild type) | PCR ribotype 012 | 18 |
| ISST82Ia, ISST82Ib, ISST82Ic | 630 gyrA(Thr-82→Ile) | This study |
| ISST82Va, ISST82Vb, ISST82Vc | 630 gyrA(Thr-82→Val) | This study |
| ISSD426Na, ISSD426Nb, ISSD426Nc | 630 gyrB(Asp-426→Asn) | This study |
| ISSD426Va, ISSD426Vb, ISSD426Vc | 630 gyrB(Asp-426→Val) | This study |
| Plasmid | ||
| pMTL-SC7315 | E. coli-C. difficile shuttle vector (pCB102 catP ColE1 traJ codA) | 7 |