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Cytotoxicity measurements of compounds F1, F12, and F19 on mouse macrophage cell line J774.2. Toxicity was assessed by measuring lactate dehydrogenase (LDH) leakage from cells at 490 nm. There is no statistically significant LDH leakage beyond that of the vehicle control for any of the three compounds up to a concentration of 200 μM. The positive control is 1× lysis control solution from the Promega CytoTox 96 assay kit, and the vehicle control is 1% DMSO, 1% ethanol, 1% Kolliphor.
