. 2015 Feb 11;59(3):1525–1533. doi: 10.1128/AAC.04261-14

TABLE 3.

Cytokine expression in blood after LiCl treatment of K. pneumoniae infection^a

Treatment	Cytokine level (pg/ml)
	18 h						72 h
	TNF-α	IL-6	MCP-1	KC	IL-1β	IL-10	TNF-α	IL-6	MCP-1	KC	IL-1β	IL-10
None	<5^b	<5^b	<10^b	22 ± 10	<5^b	<5^b	<5^b	<5^b	<10^b	22 ± 10	<5^b	<5^b
400 μg/ml LiCl	<5^b	<5^b	<10^b	23 ± 8	<5^b	<5^b	<5^b	<5^b	<10^b	25 ± 8	<5^b	<5^b
NK-9	164 ± 10	888 ± 68	954 ± 216	878 ± 49	<5^b	46 ± 11	251 ± 15	15,898 ± 99	988 ± 216	2,461 ± 98	<5^b	630 ± 55
NK-9 + 10 μg/ml LiCl	29 ± 10	364 ± 89	1,281 ± 87	350 ± 98	<5^b	12 ± 5	80 ± 11	860 ± 279	398 ± 78	521 ± 98	<5^b	23 ± 15
NK-9 + 100 μg/ml LiCl	33 ± 2	273 ± 95	868 ± 49	430 ± 49	<5^b	16 ± 3	59 ± 8	1,019 ± 106	152 ± 58	596 ± 23	<5^b	17 ± 5
NK-9 + 400 μg/ml LiCl	13 ± 3^c	90 ± 11^c	299 ± 30^c	187 ± 21^c	<5^b	<5^b	5 ± 1^c	21 ± 5^c	18 ± 4^c	53 ± 10^c	<5^b	<5^b

Groups of four mice were inoculated, via the intragastric route, with 5 × 10⁸ K. pneumoniae NK-9 cells per mouse. Various concentrations of LiCl were administered immediately postinfection by addition to the drinking water. The mice were sacrificed at 18 or 72 h postinfection, and cytokine expression in sera was detected with CBA flex sets. Data are presented as mean ± standard deviation. The statistical analysis was performed using repeated-measures ANOVA.

Minimal cytokine concentration detected using a CBA flex kit.

P < 0.05, compared with results for NK-9-treated mice.