FIG 1.
Graphical representation of FICimins determined at 10%, 25%, and 50% of growth. Five groups are presented: (i) the azole-susceptible group (wild type [WT]) with mutations neither in cyp51A nor in hapE, (ii) the azole-resistant TR group (TR34/L98H, TR46/Y121F/T289A, TR53/L98), (iii) the azole-resistant M220 group (M220V, M220K, M220I, M220R), (iv) the azole-resistant G/W group (G138C and G54W), and (v) the isogenic group consisting of the two azole-resistant R1 and R2 strains with substitutions in hpaE. The parental S1 and S2 azole-susceptible strains from R1 and R2 were added to the WT group. Significant differences were found at the 50% growth endpoint, with the strongest synergy found for isolates harboring the M220 mutation.